Phis2自激活
WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html
Phis2自激活
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http://biotech.aiijournal.com/CN/10.13560/j.cnki.biotech.bull.1985.2024-0917 Webb8 juli 2024 · pHis2 编号 载体名称 北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息 出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体 高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点,限制性内切酶 载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签 ...
Webb24 apr. 2016 · Figure 酵母单杂和双杂筛选的一般步骤,更加详细单杂和双杂筛选流程图,请参考Figures BDMatchmaker TM 单杂和双杂文库筛选与构建. 如这个图表所示,As diagramshows,重组介导克隆使文库构建和筛选更快捷高效, 尽管这里没有显示,双杂文库能通过酵母融合筛选(细节 ... Webb先下结论,不存在可以预测蛋白是否互作的预测方法,小分子和蛋白互作可以通过计算MM-PBSA结合自由能来预测,蛋白-蛋白是否互作需要借助实验手段如IP、等温滴定量热(ITC)或者表面等离子共振(SPR)。. 有了实验基础,可以通过模拟的方式预测蛋白的结 …
Webb使用phis2载体,将启动子序列构建在his3报告基因前面的多克隆位点上,对应的转录因子构建在ad载体上即可。使用y187菌株,它相对于ah109菌株在基因组上少了his的报告基 … Webb6 sep. 2024 · 1)pHis2-bait和AD-prey质粒混合作为实验组,将pHis2-bait和空载AD质粒混合作为自激活组。 将实验组和自激活组分别共转到Y187感受态细胞中。 2)用SD/-Leu/ …
WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ...
http://www.kelei-biology.com/plus/view.php?aid=1071 slow cooker cranberry saucehttp://www.zoonbio.com/molecular/yeast-one-hybrid.html slow cooker cranberry catalina chickenhttp://www.bioon.com.cn/doc/showarticle.asp?newsid=101857 slow cooker cranberry recipesWebbY187-pHis2YeastOne-HybridMediaKit(酵母单杂培养基套装YM1010),含有用于Y187-pHis2系统 酵母单杂交文库筛选的全套培养基,性价比更高。培养基以非常方便使用的 … slow cooker crack slawWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple cloning site ... slow cooker crack potato souphttp://www.biogenesci.com/ slow cooker creamed corn damn deliciousWebb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and consistent clones were diluted, ... slow cooker creamed corn just like rudy\u0027s bbq